Biological buffer Tris for life science research

Details:

As we all know, biological buffer is often used in biological experiments to maintain the pH of the experimental system. When a certain amount of acid and alkali is added to some solutions, it can hinder the change of pH of the solution, which is called buffering. Therefore, this kind of solution is called biological buffer solution. There are many kinds of biological buffers, so which one should be selected for life science research I believe many people don't know. Next, let's learn about it Tris buffer.

The buffer system suitable for life science research shall have the following characteristics:

① PKa value is between 6-8;

② High solubility in water;

③ Difficult to penetrate biofilm;

④ Salt effect is small;

⑤ Ion concentration, solution composition and temperature have little effect on dissociation;

⑥ Do not form complexes or precipitates with metal ions;

⑦ The buffer is chemically stable;

⑧ The light absorption is small in the wavelength range of ultraviolet and visible light;

⑨ It is easy to make high purity salt.

The common effective pH range of Tris buffer is in the "neutral" range, for example:

Tris HCl buffer: pH = 7.5-8.5

Tris phosphate buffer: pH = 5.0-9.0

Tris HCl buffer: because Tris is highly alkaline, this buffer system can be used to prepare a buffer with a wide range of pH from acidic to alkaline. It has high solubility in water and is inert to many enzymes. It has little interference with biochemical process, and does not precipitate with calcium, magnesium ions and heavy metal ions. Therefore, Tris buffer is used more and more in biochemical research, but it should be noted that Tris HCl buffer prepared at room temperature cannot be used at 0 ℃ - 4 ℃; Moreover, it is easy to absorb CO2 in the air, so the prepared buffer should be tightly sealed.

Desheng has been producing and developing biological buffers for more than ten years. Tris series products have a professional R & D team. At present, the products have been sold to more than 100 countries around the world, with guaranteed product quality and worry free after-sales. If necessary, please feel free to contact us at any time and look forward to your call!