What are the configuration methods of Tris HCl solution?

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 Tris HCl  is fully known as trimethylolaminomethane hydrochloride in Chinese and Tris hydrochloride in English. I believe many people are familiar with tris. Tris is widely used. Tris buffer is not only used as a solvent for nucleic acids and proteins, but also has many important uses.

Tris was used for protein crystal growth under different pH conditions. The low ionic strength of Tris buffer can be used for the formation of intermediate fibers of nematode lamin. Tris is also one of the main components of protein electrophoresis buffer. It forms a buffer system with glycine in the electrophoresis buffer to stabilize the pH in the electrophoresis process. In addition, Tris is also an intermediate for the preparation of surfactants, vulcanization accelerators and some drugs. Tris was also used as a titration standard. As a protein buffer, if mass spectrometry is required for subsequent work, Tris is not suitable. Try to replace it with a buffer that can be tolerated by other mass spectrometers.

Tris HCl is obtained by the reaction of Tris as the main raw material. Its solution is acidic and is mainly used as a biological buffer to adjust the pH value of virus preservation solution. If it needs to be adjusted to neutral or alkaline, Tris or NaOH can be added. Apart from this information, what are the configuration methods of Tris HCl How much do you know The configuration method of Tris HCl is described below.

Preparation method of Tris HCl buffer:

1、Tris-Hcl（0.05mol/L，25℃）

Mix 50ml 0.1mol/l Tris solution with X ml 0.1mol/l hydrochloric acid, and dilute to 100ml with water

PH x/ml PH x/ml

7.1 45.7 8.1 26.2

7.2 44.7 8.2 22.9

7.3 43.4 8.3 19.9

7.4 42.0 8.4 17.2

7.5 40.3 8.5 14.7

7.6 38.5 8.6 12.4

7.7 36.6 8.7 10.3

7.8 34.5 8.8 8.5

7.9 32.0 8.9 7.0

Tris molecular weight = 121.14:0.1mol/l, and the solution is 12.114g/l. Tris solution can absorb carbon dioxide from the air. The bottle cap should be tightly closed when using. When Tris HCl solutions with different concentrations and pH values are prepared, they can be prepared into a 0.05mol/l solution with a certain pH value, and then diluted to the required concentration.

2、1M Tris-Hcl（PH7.4、PH7.6、PH8.0）

Component concentration 1m Tris HCl

Configuration quantity 1L

Configuration method:

1、 Weigh 121.1g Tris and place it in a 1 l beaker;

2、 Add about 800ml of deionized water and fully stir to dissolve;

3、 Add concentrated HCl according to the following values to adjust the required pH value;

PH concentrated HCl

7.4 about 70ml

7.6 about 60ml

8.0 approx. 42ml

4、 Dilute the solution to 1L;

5、 After high temperature and high pressure sterilization, store at room temperature.

3、1.5M Tris-Hcl（PH8.8）

Component concentration 1.5m Tris HCl

Configuration quantity 1L

Configuration method:

1、 Weigh 181.7g Tris and place it in a 1L beaker;

2、 Add about 800ml of deionized water and fully stir to dissolve;

3、 Adjust the pH value to 8.8 with concentrated HCl;

4、 Dilute the solution to 1L;

5、 After high temperature and high pressure sterilization, store at room temperature.

It should be noted that the pH value of Tris solution should be adjusted after the solution is cooled to room temperature, because the pH value of Tris solution varies greatly with temperature. The pH value of the solution decreases by about 0.03 units every 1 ℃ increase in temperature.

The above is the configuration method of Tris HCl provided by Hubei xindesheng Materials Co., Ltd. Desheng produces other chemicals besides Tris HCl. At present, Desheng company has been established for decades, has its own R & D team, and has invested a lot of energy in biological buffers. Product quality is reliable, price concessions can be discussed. If you are interested in understanding, you can call for consultation. Desheng welcomes your call.