Advantages and disadvantages of Tris buffer and its derivative buffer function

Details:

Buffer solution is usually a solution composed of "weak acid and its conjugated base" or "weak base and its conjugated acid" buffer pairs, which can slow down the change of pH when adding a certain amount of other substances. The function of buffer is to adjust the pH value of the solution within a limited range so that the acidity of the solution to be tested meets the range specified by the analysis method. In biochemical experiments and related research work, a large amount of buffer is often needed to maintain the experimental body

PH of the system. Tris and its derivative buffer are widely used in organic synthesis, coatings, fireproof materials and raw materials

Chemical and pharmaceutical fields are widely used, so what are the advantages and disadvantages in practical application

Firstly, Tris buffer Tris, also known as trimethylolaminomethane, is more and more widely used, especially in the research experiments related to nucleic acid and DNA, which has more advantages than PBS phosphate buffer. On the other hand, Tris is also an important raw material for the synthesis of another biological buffer taps.

Tris has obvious advantages. Its pH buffer range is 7-9 and its alkalinity is strong. This kind of buffer can be used only

PH buffer in the range from acid to alkali is configured for the flushing solution; At the same time, because it does not precipitate with heavy metals, it can be one

It works and has little interference with biochemical processes. Therefore, Tris buffer is not only widely used as nucleic acid and egg

White matter solvent, there are many important uses. The low ionic strength of Tris buffer can be used for the formation of intermediate fibers of lamin in C. elegans. Tris is also one of the main components of protein electrophoresis buffer. It forms a buffer system with glycine in the electrophoresis buffer to stabilize the pH in the electrophoresis process. Tris buffer also has some disadvantages to be noted: 1. The pH of Tris buffer is greatly affected by the concentration; 2. It is greatly affected by temperature effect. For example, Tris buffer with pH of 7.8 at room temperature of about 25 ℃ is 8.4 at 4 ℃ and 7.4 at 37 ℃. Therefore, when the buffer prepared at 4 ℃ is measured at 37 ℃, its hydrogen ion concentration increases 10 times. And it is at pH 7 Below 5, the buffer capacity is very poor.

Several common Tris derived buffers

1. TBS buffer is mainly composed of Tris, sodium chloride, BSA, preservative, etc., pH7 4. Yes

Isotonic buffer salt solution commonly used in physics is mainly used in immunohistochemistry, in situ hybridization, enzyme-linked immunosorbent assay and other experiments to clean nonspecific bound antibodies in Western blot experiments.

2. TBS is Tris HCl buffer salt solution. It is isotonic salt solution plus Tris HCl buffer. It is mainly composed of Tris HCl, NaCl and Tween20, pH 7 2-7.5. It is mainly used to clean the immunoblot in immunohistochemistry, in situ hybridization, enzyme-linked immunosorbent assay and other experiments.

3. Te buffer is prepared by Tris and EDTA, an electrophoretic buffer, pH8 0. It is mainly used to dissolve nucleic acids and can stably store DNA and RNA.

4. Tae buffer is a buffer composed of Tris, acetic acid and EDTA, pH7 2-8.4, is a widely used buffer system for short-time electrophoresis of large fragments of DNA;

5. Tbe buffer is a buffer solution composed of Tris base, boric acid and EDTA (ethylenediamine tetraacetic acid). TBE buffer is usually used in agarose gel electrophoresis to analyze DNA products produced by PCR amplification, DNA purification protocol or DNA clone.

Hubei xindesheng is specialized in biological buffer, blood collection additives, chemiluminescence reagents and luminescence

A high-tech company integrating R & D, production and sales of substrates and other products. After 15 years of rapid development, Desheng has a huge R & D team and experimental base. Now biological buffer products not only have a high share in the domestic market, but also have been sold to dozens of countries around the world. Desheng has established long-term and stable cooperation with many large biomedical technology enterprises.