Principle and application of potassium EDTA

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There are many kinds of blood anticoagulants in blood collection reagents, and one anticoagulant is widely used, that is, EDTA potassium salt. There are many types of EDTA potassium salt, of which dipotassium is used more, but what is its principle and application There should be some people who don't know it very well. Here we can take a look at its application fields and principles.

EDTA dipotassium salt, also known as dipotassium ethylenediamine tetraacetate dihydrate (EDTA-K2), is a white crystal powder, which is easy to dissolve in water and absorb moisture, with a molecular weight of 404.6. Deta dipotassium salt can protect the cellular components of blood, does not affect white blood cells, has the least impact on red blood cells, and can inhibit the aggregation of platelets. It is suitable for general blood testing. However, in addition to the separation test of platelets, it is not suitable for coagulation test and platelet kinetic energy test, nor for the determination of calcium ion, potassium ion, sodium ion, iron ion, alkaline phosphatase, creatine kinase and leucine aminopeptidase and PCR test.

EDTA two potassium salts are widely used in complex metal ions and metal separation, as well as washing powder, shampoo, liquid soap, agricultural chemical spray, blood anticoagulant and other fields. EDTA dipotassium salt is also recommended by the International Committee for hematological standardization as the preferred anticoagulant for blood cell counting and classification.

Firstly, we understand the principle of blood coagulation, which can be divided into three parts: 1. The formation of prothrombin activator; 2. Prothrombin activator changes prothrombin into active thrombin with the participation of calcium ion; 3. Soluble fibrinogen changes into insoluble fibrin under the action of thrombin. Fibrin is like filaments, crisscrossed, netting a large number of blood cells to form jelly like blood clots. This is the process of coagulation. EDTA dipotassium salt has a great affinity for calcium ions in the blood, and can complex with calcium ions, so that prothrombin cannot be transformed into thrombin, so as to make the blood anticoagulant.

Hubei xindesheng Material Technology Co., Ltd. is a manufacturer of blood collection additives. At present, there are many kinds of blood collection additives. Many products, including potassium salt, are in stock, and the batch is stable and the product use effect is good. If necessary, you are welcome to consult Desheng's official website at any time