How to obtain high quality Tris HCl buffer

Details:

Tris HCl is a zwitterionic biological buffer with the appearance of white crystalline solid. Tris HCl has good water solubility and is colorless and clear when dissolved in water. It is often used in molecular biology research, such as electrophoresis experiment, DNA purification experiment and stabilizing the pH value of aqueous phase environment. It's easy to buy Tris HCl buffer on the market today, but you can also make it yourself with appropriate equipment.

Preparation materials:

Calculate the amount of each item required according to the concentration of the required solution and the amount of buffer required.

1. Trimethylolaminomethane (Tris)

2. Distilled deionized water

3. Hydrochloric acid

Preparation steps:

1. First, determine the concentration (molar concentration) and volume of Tris buffer to be prepared. For example, the concentration of Tris buffer solution for saline varies from 10 to 100 mm. Once the substance to be prepared is determined, the number of moles of Tris required can be calculated by multiplying the molar concentration of the buffer by the volume of the prepared buffer. (moles of Tris = mol / L x L)

2. Next, determine how many grams of Tris this is by multiplying the number of moles by the molecular weight of tris (121.14 g / mol). Tris g = (mol) x (121.14 g / mol)

3. Dissolve Tris in distilled deionized water to 1 / 3 to 1 / 2 of the final volume required.

4. Mix in HCl (e.g. 1m HCl) until the pH meter provides the required pH for your Tris buffer solution.

5. Dilute the buffer with water to reach the required final solution volume.

After preparing the solution, it can be stored in a sterile position at room temperature for several months. Tris HCl buffer solution has a long shelf life because it does not contain any protein.

At present, there are not many professional manufacturers of Tris HCl buffer in the market, and our company is one of the very professional Tris HCl manufacturers in China. Our products have transactions in many regions around the world and are widely praised by the industry.

Hubei xindesheng Material Co., Ltd. Desheng company specializes in the production of biological buffers. Since its establishment, it has produced biological buffers for more than ten years. It has independently developed Tris, mops, HEPES, taps and other products, and has a professional team to manage the R & D and production. If you are interested in Desheng's products, you can enter its official website to contact customer service for details.