The difference of nucleic acid detection vs antibody detection and the role of virus transport media

Details:

There are two ways to detect COVID-19, nucleic acid detection and antibody detection. However, we generally say that nucleic acid detection naturally ignores antibody detection. This is because antibody detection is generally used as a supplementary detection index for suspected cases with negative coronavirus nucleic acid detection, or used in conjunction with nucleic acid detection in the diagnosis of suspected cases, which can not be used as the basis for diagnosis and exclusion, and is more suitable for retrospective examination. What is the difference between the two new crown tests What kind of test is more suitable for disease virus preservation solution

Virus transport media is a liquid used for disease virus sample detection. There are mainly inactivated type and non inactivated type. Inactivated type can quickly inactivate disease virus and release disease virus nucleic acid for rapid detection of disease virus nucleic acid; The non inactivated type can protect the activity of disease virus RNA and protein at the same time, which can be used for disease virus nucleic acid detection and antibody detection.

Differences between nucleic acid detection and antibody detection:

Different samples

Nucleic acid detection requires respiratory tract samples, including pharynx, nasopharynx secretion, sputum, bronchus, lavage fluid, lung tissue biopsy, etc. the collection process is very complex.

Antibody test is a blood test test, which can collect blood through terminals or veins. The sample collection method is more convenient and safe, and the sample can be stored for 72 hours.

Different detection methods

Nucleic acid detection is a direct detection of disease virus. It takes the specific DNA sequence of disease virus as the detection target. Through PCR amplification, the DNA sequence of this target selected by us increases exponentially. Each amplified DNA sequence can be combined with a section of fluorescent labeled probe added in advance to produce fluorescent signal. The more target DNA amplified, the stronger the cumulative fluorescence signal. In the samples without disease virus, the fluorescence signal enhancement could not be detected because there was no target DNA amplification. Moreover, the nucleic acid sample has harsh storage conditions, the RNA is very easy to crack, and can only be stored for 24 hours at 4 ℃.

Antibody testing is an indirect test. It is not aimed at the disease virus itself, but the specific antibody produced by the human immune response after being infected with the disease virus. About a week after the human body is infected with the disease virus, the antibody is gradually produced and then rises rapidly. The antibody called IgM is generally produced in the human body first. The positive IgM antibody indicates the recent infection, and the positive IgG antibody indicates the long-term or past infection.

Different detection conditions

Nucleic acid detection requires special instruments and standard molecular detection laboratories, and requires PCR certified personnel to operate. The steps are cumbersome, and the whole process takes 5 ~ 8 hours, resulting in a high risk of contamination in the process.

Antibody detection antibody detection breaks through the restrictions of existing nucleic acid detection methods on personnel / places. It can be detected quickly and in large quantities, and can be completed in grass-roots laboratories with low risk of contamination.

It can be seen that no matter which new coronavirus detection method has advantages and disadvantages. Antibody detection is a supplement to the "false negative" of nucleic acid detection. The combination of the two can effectively improve the diagnostic efficiency of Xinguan, which is of great value for epidemic prevention and control.

Hubei xindesheng Material Technology Co., Ltd. is a manufacturer specializing in the production and sales of virus transport media. If you need to buy, you are welcome to inquire and buy at any time!