Application of 4-hydroxyethyl piperazine ethanesulfonic acid

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The main component of HEPES buffer is hydroxyethyl piperazine ethylthiosulfonic acid. HEPES is a non-ionic amphoteric buffer, which is at pH 7 2-7.4 it has good buffer capacity in the range of. It is widely used in cell culture medium, protein, IVD kit and other fields. It can adjust the pH value of the solution from 6.8 to 8.2, which is especially suitable for pH adjustment under physiological conditions. In addition to the above applications, it is also used in cosmetic formulas. Most culture media contain phenol red as pH indicator, acid culture media are orange yellow and alkaline culture media are dark HEPES is somewhat expensive and may be toxic to some cells at high concentrations In principle, HEPES buffer can be used to replace bicarbonate to remove the limitation of high concentration CO2 culture environment In practice, it is not so simple. Dissolved CO2 and bicarbonate are also important for good cell growth.

In cosmetic solution, HEPES is used to balance the properties of its acidic counterpart. HEPES can keep the pH constant in the process of chemical reaction, so it is often paired with components such as glycolic acid, which helps to prolong and maintain the quality of skin care products. Ingredients that provide exfoliation to improve the appearance of the skin are usually selected.

Desheng biochemical is a professional manufacturer of biological buffer, with many kinds of products. Since its establishment in 2005, it has more than ten years of R & D and production experience. The HEPES buffer produced by Desheng has high purity and wide application range. If you need anything, please contact us!