Buffer Tris in protein purificationMain components of cell lysis buffer Tris-HCl and EDTA

Details:

Lysis refers to a state of cell division or rupture. Lysis buffer is a solution that helps to open cells. It can be used when some experimental analysis needs to extract cell contents. For example, scientists must use lysis buffer to assist in extracting DNA or protein from cells for analysis. There are many kinds of cell lysis buffer, among which the more common one is Tris-HCl buffer.

Tris-HCl can maintain the best pH value of cell lysis buffer, so it is often used as the main component of lysis buffer. Lysis buffer helps to open cells and screen substances in cells, such as salts, cleaners, chelators and inhibitors, as well as some alkaline chemicals. It can not only distinguish and screen, but also remove these substances.

In addition to the biological buffer, the lysate also includes a chelating agent and inhibitor, such as ethylenediamine tetraacetic acid (EDTA), because this chemical combines with two positively charged metal ions (such as magnesium and calcium), which can prevent them from being used in other reactions. Many DNases (proteins that chew DNA) and proteases (proteins that chop other proteins) require magnesium ions to function, so EDTA helps to reduce the activity level of proteases or DNases by depriving these key components.

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