The role of serum separation gel in clinical testing and the reasons for its poor effect

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I believe many people don't understand the product of serum separation gel, but many people have been exposed to it, especially when we go to the hospital for blood collection testing. The additive separating glue in the blood collection vessel can effectively shorten the blood coagulation time. But what exactly does it do How is it used in clinical testing Let Desheng, a manufacturer with more than ten years of R & D and production experience, answer for you.

Principle of serum separation gel

Serum separation gel can form an isolation layer between cell components and serum, effectively prevent material exchange between blood cells and serum, and ensure the stability of serum components within a certain period of time.

Separation process of serum gel

The mechanism of separation gel in separating serum and plasma is that the separation gel itself is composed of hydrophobic organic compounds and silica powder. It is a thixotropic mucus. The colloidal structure contains a large number of hydrogen bonds. The existence of this substance constitutes the basis of thixotropy of the separation gel. When the separation gel and coagulated blood are centrifuged in the same test tube, Due to the centrifugal force exerted by the separation gel, the hydrogen bond network structure is destroyed, and the network structure becomes a chain structure, and the separation gel becomes a fluid with low viscosity. Due to different specific gravity, the separation gel changes, and the three layers of blood clot, separation gel and serum are formed in layers. When the centrifuge stops centrifugation, the chain particles in the separation gel will form a network structure again, and an isolation layer will be formed between serum and blood clot.

Reasons for the poor effect of serum separation gel in clinical test

1. Quality of separating glue

The specific gravity of separating gel is between serum and blood cells, which is the physical basis of the reversibility of separating gel and separating serum. If the quality of blood collection gel is poor and the specific weight can not meet the requirements, it is bound to affect the effect of serum separation, and it is easy to interweave the gel and serum.

2. Incomplete blood coagulation

After centrifugation, sometimes the separation of rubber barrier and serum and blood clot is incomplete, and fibrin filaments appear in serum. The reason is often that the blood does not coagulate completely before centrifugation. Incomplete blood coagulation can make fibrin mixed in the isolation layer.

3. Centrifugal temperature

The centrifugation temperature has a significant effect on the separation of serum. The serum was clear in the inert separation gel promoting tube separated by ordinary centrifuge at room temperature, but oily beads of different sizes appeared in 15% ~ 20% of the samples. No oily beads were found in the serum isolated from the test tube centrifuged by low-temperature centrifuge. When the temperature exceeds the storage temperature required by the separation hose, the inert separation gel will dissolve in the serum.

4. Centrifugal operation

Centrifugation is an important link in preparing high-quality serum samples. If the centrifugal force is too low, the force acting on the separation gel is weak, resulting in poor reversibility of the separation gel. Fibrin condensate can stay in the serum or colloidal layer, which is easy to block the sample needle of the automatic analyzer. On the contrary, if the centrifugation time is too long, it is difficult to form a network structure after the hydrogen bond is cut off in the separation gel condensate, resulting in the loss of thixotropy.

5. Serum / plasma density

The density of serum is the main reason affecting the floating of separation gel, not viscosity. Any factor that leads to the increase of serum (plasma) density and exceeds the density of separation gel can cause the abnormal reversion of separation gel.

Desheng company is a professional manufacturer of blood collection additives. In addition to serum separation gel, it also produces a series of blood collection additives. If necessary, you are welcome to enter Desheng's official website at any time for product details!