TES and BIS Tris derivatives of biological buffer Tris

Details:

Tris is a commonly used biological buffer, which is widely used in the field of biochemistry. At the same time, it is also an important chemical raw material. For example, the common Tris HCl, TAE, TES, BIS Tris, etc. are synthesized from it. This paper will introduce TEs and BIS Tris derivatives in detail.

1. Tes (tris ethanesulfonic acid)

Chinese name of TEs: tris ethanesulfonic acid, CAS No.: 7365-44-8. The structure of TEs is similar to trizma. It is one of the series of ethanesulfonic acid physiological buffers developed by good and meets various standards of "good" buffer. Although the structure is neutral molecule, it is still zwitterionic in the solution. The pKa of TEs is 7.4 (physiological pH), so it is a widely used biological buffer reagent.

TES is suitable for various cell culture systems that need divalent metal ions, while many other buffers (such as citrate or phosphate) will produce chelation or precipitation reaction, which makes it unable to meet this requirement. In addition, TES is very helpful to study the oxidation reaction of succinic acid.

2. BIS tris (BIS trimethyl hydrochloride)

BIS Tris Chinese Name: bis (2-hydroxyhexyl) amino (trimethylol) methane, CAS No.: 6976-37-0, is an inert zwitterionic buffer, and its dissociation constant changes only slightly with the change of temperature. Bicine is suitable for the ionization of trace hydrogen ions in the acidic system, and bicine has the ability to introduce more electrons to the weak metal ions, which is similar to that of EDTA.

BIS Tris is suitable for the separation of hemoglobin. It can protect the stability of hemoglobin during freeze-drying preservation. It can be used as an effective buffer for muscle enzyme activity analysis. 2-D gel electrophoresis is used as cathode fluid in isoelectric focusing electrophoresis (IEF).

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