Application and precautions of acridine ester series products

Details:

Among various non radioactive immunoassay methods, chemiluminescent immunoassay (CLIA) has the advantages of high sensitivity, wide detection range, good precision, stable marker, full automation and wide application. The characteristics of CLIA with acridine ester derivatives as luminescent markers are: simple marking, stable marker, no catalyst, low natural background The interference factors are less, the signal-to-noise ratio is high, and the light-emitting system is simple.

Acridine ester is a general term of acridine salt, which refers to a kind of chemical substances that can be used as chemiluminescence markers. Acridine ester has several different series, including acridine salt DMAE NHS, acridine ester NSP DMAE NHS, acridine salt NSP SA, etc. the synthesis method, performance and advantages of acridine ester DMAE NHS are introduced.

The synthesis route of acridine DMAE NHS is as follows:

1. the crude product was obtained by dissolving 4-hydroxy-3,5-dimethylbenzoic acid and N-hydroxysuccinimide in N, N-dimethylformamide (DMF), and then the crude product was obtained by the reaction of the condensation agent;

2. the crude product was obtained by heating reaction under the action of catalyst, and the crude product was separated and purified by column chromatography.

3. the product obtained by 2 was dissolved in anhydrous toluene with dimethyl sulfate. The mixture was filtered, the filter residue was washed and recrystallized to obtain DMAE NHS, the target product.

The introduction of the luminescent properties of DMAE · NHS

1. the study on the luminescent properties of DMAE NHS shows that the light emission of DMAE NHS is flash, the maximum luminescent intensity is reached at 0.4s, the half-life of DMAE NHS is about 0.9s, and the luminescent intensity is 6.11 × 1018cps/mol。 The composition of the luminescent starting agent has an effect on the luminescent intensity. Surfactants such as CTAC, TritonX-100, Tween-20 can enhance their luminescence.

2. The luminescent properties of DMAE NHS and antibody coupling DMAE NHS AB are similar. When the molecular ratio of DMAE NHS to AB is 2.4, the luminescent intensity is 1.8 × 1019cps/molAb。 DMAE NHS and DMAE NHS AB have good thermal stability. In Pb buffer solution pH7.0 at 4 ℃ or frozen, their luminescent activity or immune activity basically did not decrease after half a year. At room temperature or 37 'C, the luminescent activity decreased due to hydrolysis. Their thermal stability decreased with the increase of temperature, and decreased with the increase of pH value of molten liquid,

Advantages of DMAE NHS

The results show that DMAE NHS has good luminescent properties, easy to be coupled with antibody, stable coupling, simple luminescent system, and is an ideal chemiluminescent immunoassay marker. The tshcalia, tshbas CLIA and tt4bas CLIA methods, which are established by DMAE NHS as luminescent markers, have good linearity, high signal-to-noise ratio, wide detection range and high sensitivity, The accuracy, accuracy and soundness of IRMA and cibacomingclia were well correlated.

Acridine ester has obvious advantages in in vitro diagnosis, and has better sensitivity. At present, the application of acridine ester chemiluminescence immunoassay technology has gradually developed into a trend in the fields of medicine detection, diagnostic analysis, environmental detection, food detection and so on. Desheng technology provides reliable acridine ester and acridine salt products, which can meet the scientific research and production needs of scientific research institutions and reagent box manufacturers.