Detailed configuration steps of Tris saturated phenol

Details:

Tris full name: trimethylolaminomethane, as a common chemical, is widely used in various fields. For example, Tris can be seen in the fields of medicine, daily chemicals, special materials and so on, in which there is an application, "nucleic acid purification". Nucleic acid purification requires saturated phenol. Tris also has saturated phenol, also known as basic phenol and Tris equilibrium phenol. Tris saturated phenol is tris clph8 0 saturated re evaporated phenol is a light yellow transparent liquid with pungent smell. The upper layer is Tris hydrochloric acid buffer, and antioxidant 8-hydroxyquinoline is added to the solution. It is suitable for removing protein and extracting DNA from nucleic acid samples. Although there are ready-made products on the market, it is better to master the configuration method of Tris saturated phenol. The detailed configuration steps of Tris saturated phenol are described below.

Put the phenol in the refrigerator at room temperature for 1 s to prevent the phenol from cracking, and put it into the water bath at a temperature of 68 ℃ to prevent the phenol from cracking immediately; 2. Add 8-hydroxyquinoline to 0.1% and β－ Mercaptoethanol to 0.2% (stock solution 14.4mol / ml), mix well, the solution appears yellow, and pour it into the separating funnel (it can also be carried out in the beaker); 3. Add 1mol / ml tris (ph8.0) of equal solvent, mix it repeatedly, and then let it stand until it is layered; Release the lower Yellow phenol solution and discard the upper layer; 4. Add about 1g / 100ml of solid Tris phenol, shake well and remove the aqueous phase; 5. Add 0.1mol/mltris (ph8.0) to balance several times until pH is 8.0; 6. Add 0.1mol/mltris (ph8.0) to the brown bottle for 4 degrees preservation; 7. If yellow disappears or turns pink, it cannot be used.

Tris saturated phenol is toxic and has strong irritating effects on skin and human body. It needs to be operated in the fume hood. Avoid hazards and put safety first.

The above is the configuration method of Tris saturated phenol provided by Hubei xindesheng Materials Co., Ltd. In addition to producing Tris, Desheng also produces other chemicals, such as blood collection additives, enzyme preparations, chemiluminescence reagents, biological buffers, chromogen substrates and carbomer. At present, Desheng company has been established for decades, has its own R & D team, and has invested a lot of energy in biological buffer. Product quality is reliable, price concessions can be discussed. If you are interested in understanding, you can call for consultation. Desheng welcomes your call.