How much do you know about the application and configuration methods of caps

Details:

The full name of CAPS is 3-cyclohexylaminopropanesulfonic acid. It is white powder crystal particles and can be dissolved with water, but the dissolution rate is slow. CAS number is 1135-40-6 and the molecular formula is c9h19no3s. It belongs to biological buffer and is mostly used in biochemical diagnosis kit, PCR diagnosis kit, DNA extraction kit and RNA extraction kit. But what other applications are there besides these The applications of CPSA are described below.

1. Caps is used as a biological buffer: caps is mainly used in biochemical diagnostic kit, DNA / RNA extraction kit and PCR diagnostic kit as a buffer for enzyme chemistry and HPLC separation of alkaline drugs. Caps can also support alkaline phosphatase activity and inhibit the growth of Aeromonas at pH 10.5, and dissolve in deionized water and adjust pH to 11.0 for the purification of fibronectin.

When caps is used as a biological buffer, it needs better purity. Generally, it needs to analyze the purity. When it is used in industry, the purity requirement is relatively low, but it also needs to be treated differently for different applications.

2. In industry, caps is used for new coatings and new materials. Caps is also the raw material for manufacturing welding materials, air conditioning equipment and metal lithium.

3. It is used as analytical reagent, heat exchange carrier and pharmaceutical industry. It is also used for air conditioning, fireworks, dry batteries and metal lithium. It is also used as flux and desiccant.

4. For the coating industry, it is used as waterborne isohydrogenate curing agent. Aqueous isocyanate curing agent can coexist stably with resins containing active groups (hydroxyl, carboxyl, amino, epoxy, etc.) at room temperature for a long time.

This is the general application of caps. The following will introduce the general configuration method of caps

Since caps buffer is mainly composed of caps and deionized water, adjust the pH value with sodium hydroxide to avoid contact with air. Add deionized water into caps to 900ml, adjust the pH value to 11.0 with 2mol / L NaOH, then fix the volume to 1L and store it at 4 º C. Generally, the buffer with high methanol concentration has a good effect on the transfer of low molecular weight proteins, while the buffer with low methanol concentration or even without methanol is conducive to the transfer of high molecular weight proteins. The concentration of methanol in caps electroimprinting buffer is 0 ~ 20%, so caps electroimprinting buffer is more suitable for the transfer of low molecular weight proteins.

Hubei xindesheng materials is specialized in the production and R & D of biological buffer and blood collection additives, enzyme preparations, chemiluminescence reagents, luminescent substrates and other products. Desheng has been established for decades, has its own R & D team, and has developed biological buffers for many years. Its products include Tris, HEPES, taps, mops, caps, bicine, EPPs, pipes and a series of biological buffers. At present, Desheng's products have been sold to many countries all over the world, received high praise and re purchased, and reached long-term cooperation with many enterprises. If you are interested in understanding, you can call for consultation. Desheng welcomes your call.