Effect of EDTA dipotassium pollution on the detection of heparin blood biochemical items

Details:

Ethylenediamine tetraacetic acid (EDTA) is a commonly used anticoagulant for testing, including disodium (edta-na2), dipotassium (EDTA-K2) and Tripotassium (edta-k3). Blood routine examination is a common blood test item. In order to ensure that the blood does not coagulate during blood drawing, blood collection vessels containing anticoagulants are currently used. The commonly used anticoagulant is EDTA-K2, which has a good anticoagulant effect and little impact on the morphology of blood cells.

Because the solubility of potassium salt is higher than that of sodium salt, EDTA-K2 and edta-k3 are better than edta-na2; Because the pH of EDTA-K2 is lower than that of edta-k3, it can compensate for the cell shrinkage caused by osmotic pressure, so it can be removed. EDTA-K2 pollution often causes the abnormal increase of K concentration in patients, which is inconsistent with the clinical manifestations of patients. However, EDTA-K2 pollution may also affect other biochemical indexes, but how much EDTA-K2 concentration affects which conventional biochemical indexes This Lippi on Biochem MED (Zagreb) G team's article systematically discussed the impact of the increase of attached EDTA-K2 concentration on the detection results of routine biochemical indexes.

Materials and methods: 15 volunteers from our laboratory were recruited into the study. Each Research Institute

Two tubes of heparin lithium and one tube of EDTAK2 samples were collected. EDTA whole blood was diluted with heparin lithium blood to contain EDTA

Heparin lithium samples at concentrations of 0%, 5%, 13%, 29% and 43%. Next, separate the samples and detect the biochemical indexes: alt, total bilirubin, total cholesterol, creatinine, iron, LDH, lipase, potassium, sodium, chlorine, magnesium and phosphorus. Results: with the increase of edta2k concentration (from 5%), the detection results of calcium, cholesterol, iron, lactate dehydrogenase and magnesium decreased, but potassium increased. The EDTA concentrations that significantly increased the detection results of sodium phosphate were 13% and 29%, respectively. The results of alt, total bilirubin and lipase did not change with the increase of EDTA concentration. Low concentrations of edta2k (from 5%) had a greater effect on calcium, cholesterol, lactate dehydrogenase, magnesium and potassium. For sodium phosphate iron, it needs a high edta2k (from 29%) to have an effect.

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