Effect of ethylenediamine tetraacetic acid (EDTA) dipotassium on anticoagulant in vitro

Details:

In some clinical tests in the hospital, whole blood or plasma examination is used. Venous blood is usually collected for examination samples. Different anticoagulants will also be added according to different examination needs. In the specific field of in vitro diagnosis, anticoagulants are usually added to the blood collection vessels to prevent blood coagulation from forming blood clots, so as to maintain the fluidity of blood and facilitate blood testing. Ethylenediamine tetraacetic acid (EDTA) is a kind of polybasic acid, which contains four carboxylic acid groups and two amine groups. It has lone pair electrons and can chelate calcium and several other metal ions. Calcium is an essential substance for various enzyme reactions in the coagulation cascade. EDTA combines with calcium ions to form chelates. The removal of calcium can effectively prevent blood coagulation in the collection tube.

Ethylenediaminetetraacetic acid (EDTA) has always been recommended as the preferred anticoagulant for whole blood cell analysis and detection, because it can well preserve the morphology and composition of cells. In recent decades, due to the complexity of blood detection, the potential application range of EDTA anticoagulant has been significantly expanded. It can be used to stabilize blood cells tested in various experiments and protect unstable molecules.

Main uses of ethylenediamine tetraacetic acid (EDTA): it is suitable for many hematological tests, especially platelet count. EDTA-K2 is most suitable for whole blood cell analysis and hematocrit determination. The International Committee for hematological Standardization (ICSH) recommends that EDTA-K2 be used as an anticoagulant for blood cell count.

Note: EDTA can inhibit platelet aggregation and is not suitable for coagulation test and platelet function test.

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