These advantages of biological buffer bicine

Details:

Bicine ?????? chemical name is n, N-bis (hydroxyethyl) glycine (CAS: 150-25-4) and its English name is n, N-bis (2-hydroxyethyl) glycine. It is a white crystalline powder.

It is soluble in water and insoluble in acetone, DMF, DMSO, DMAC and other organic solvents. N. N-dihydroxyethyl glycine is a pH buffer suitable for biochemical and medical research. The pH buffer range is 7.6-9.0 and the pKa value is 8.3 at 25 ℃. This property makes it often used as a buffer solution for capillary electrophoresis. Its molecular structure is similar to that of glycine. It is a widely used buffer, which is not only used to prepare stable substrate solution for guaninase determination, but also used as electrophoresis buffer with working concentration of 3-100ml. Electrophoretic buffer is an important component of gel electrophoresis system for nucleic acid and protein. It is also a conductor in electrophoresis field and a necessary condition for maintaining the constant pH value of electrophoresis system.

Buffer solution refers to the mixed solution composed of weak acid and its salt, weak base and its salt, which can offset and reduce the impact of external strong acid or strong base on the pH of the solution to a certain extent, so as to maintain the pH value of the solution relatively stable. In biochemical research, buffer solution is often used to maintain the pH of experimental system.

The change of pH value of the solution system often directly affects the effectiveness of the research work. If the pH value of the "extracted enzyme" experimental system changes or changes greatly, the enzyme activity will decrease or even lose completely. Therefore, the preparation of buffer solution is an indispensable and key step. Buffer solution is an important concept in Inorganic Chemistry and analytical chemistry. Buffer solution refers to a solution that can maintain the relative stability of pH. Within a certain range, the pH value will not change significantly due to dilution or addition of a small amount of acid or alkali. The buffer solution has different pH value and buffer capacity according to the amount of conjugated acid-base pairs and their substances.

Desheng technology will continue to focus on the needs in the field of medical testing, continue to develop and research advanced biochemical reagents, so as to meet the needs of the development of global medical testing technology and strive for the cause of human health. The company customizes various ready to use solutions. Welcome to consult. PH can be customized.