PH range and advantages and disadvantages of Tris buffer

Details:

Tris buffer is a widely used buffer in biochemical research, and its commonly used effective pH range is in the "neutral" range pH=7.5~9.0

Advantages of Tris buffers
① Because of the strong alkalinity of Tris base, only this buffer system can be used to prepare buffers with a wide pH range from acidic to alkaline;
② Little interference to biochemical process, no precipitation with calcium, magnesium ions and heavy metal ions.
 
Disadvantages of Tris Buffer
① The pH value of the buffer is greatly affected by the concentration of the solution. When the buffer is diluted ten times, the pH value changes by more than 0.1;
② The temperature effect is large, and the temperature change has a great influence on the pH value of the buffer solution, △pKa/°C=-0.031, for example: the pH of the buffer solution at 4°C=8.4, then the pH at 37°C=7.4, so it must be Prepared at the temperature of use. Tris-HCl buffer prepared at room temperature cannot be used at 0°C to 4°C;
③ It is easy to absorb CO2 in the air, so the prepared buffer should be tightly sealed;
④ This buffer will interfere with some pH electrodes, so use electrodes that are compatible with Tris solution.

It can be seen that adding a small amount of strong acid or strong base to the buffer solution will not change the pH value of the solution, but if the amount of acid or alkali is added, the buffer solution will lose its buffering effect. This shows that its buffer capacity has a certain limit. The buffering capacity of a buffer solution is related to the concentration of the components that make up the buffer solution. After 15 years of independent research and development and production, Desheng Technology has been widely recognized and used by customers in the Chinese local market. Over the years, many well-known domestic manufacturers have been choosing and using Desheng products. We can also customize buffers with different pH values ??according to customer needs.