What is the principle of luminol reagent to detect blood stains?

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Luminol , a solution commonly used in crime scene testing, can identify scrubbed or long-lived bloodstains that react with blood to produce a blue glow. Luminol is extremely sensitive and is very suitable for testing the presence or absence of bloodstains in the YES/NO dimension, but it cannot guarantee that it is human blood, and criminal investigation needs to use other means to further characterize it;
According to its reaction principle, the intensity of imprinted fluorescence is proportional to the amount of blood stains without considering interfering substances. Fluorescence intensity ≠ imaging result, different exposure parameters and methods can collect different images, but generally, it is only necessary to fully expose the reaction results to easily identify the presence/absence, so the fluorescence results you generally see are mostly one. It's mushy, that doesn't mean better developing means won't lead to better pictures.
What is the principle of luminol reagent detection
Its principle is that after it is fused with hydrogen peroxide solution, it will be catalyzed by elements such as metal iron and copper, and the hemoglobin in our body contains iron, and this mixed solution even encounters very few It also glows blue when blood is present. It has also been found that although the DNA in the blood can be destroyed by strong oxidants such as potassium permanganate, the luminol reagent is different. Do identification. The luminol reagent commonly used in bloodstain detection is a mixture of luminol and hydrogen peroxide. After dissolving in an alkaline solution, it can undergo an oxidation reaction with some metal catalysts such as iron and copper. When this reaction occurs, there will be additional Energy is generated, which is emitted in the form of photons, which produces fluorescence.
There is heme in human blood, in which iron can play the role of metal catalyst, so it also emits blue-green fluorescence. Hubei Xindesheng Material Technology Co., Ltd. is a manufacturer specializing in the production of chemiluminescence reagents. It produces luminol and isoluminol, as well as acridine ester series of various luminescence reagents. You are welcome to consult and understand.