Blood Analysis Reagent Dipotassium EDTA

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Dipotassium EDTA is an anticoagulant commonly used in blood analyzers. It has the characteristics of good anticoagulation effect and less influence on the morphology of blood cells. Blood analysis is a routine test item in clinical medicine. The vast majority of outpatient or inpatient patients undergo this test. From the beginning of manual detection to fully automatic detection. All demands are also increasing.
Ethylenediaminetetraacetic acid dipotassium (EDTA-K) ethylenediaminetetraacetic acid (abbreviated as EDTA) is an amino polyacid. Because it can form complexes with most metal ions, it has become a general-purpose strong complexing agent. Past research on EDTA complexes and chelation has focused on three aspects:
1 What attracts more research forces is the most stable metal complex of EDTA - the chelate of transition elements and rare earth elements.
2 is a batch of complexes with moderate complexing strength, such as the accumulation of alkaline earth metals. Since it is difficult for general reagents to achieve the complexation of alkali metals, the complex of EDTA alkaline earth metals has attracted people's attention in particular;
3 Through the sequence of EDTA itself and co-alkali metal salts, the research on the affinity of EDTA acid radical to proton is focused.
EDTA-K is an amino polycarboxylic acid, calcium chelator, has a great affinity for Ca in blood, can effectively chelate Ca in blood samples, chelate calcium or remove calcium reaction sites, Ca content The drop will block and stop the endogenous or extrinsic coagulation process, thereby preventing the coagulation of the blood sample, and every 08mg can anticoagulate 1ml of blood. Except that it cannot be used for the determination of Ca, Na and N-containing substances in plasma, it is suitable for various anticoagulation. EDTA-K can also complex some ions in plasma to make certain proteins or nucleic acids more stable, but the interference of the formation of chromium compounds on samples and experiments should be considered.
EDTA-K is suitable for general hematological tests, especially platelet count, because it affects platelet aggregation and blood cell phagocytosis. It is not suitable for coagulation imaging and platelet function tests, nor for Ca, K, NaFe, alkaline phosphatase, creatine Kinase and leucine aminopeptidase assays and PCR assays.
Additives of vacuum blood collection tubes: The additives of vacuum blood collection tubes are ethylenediaminetetraacetic acid dipotassium (EDTA-K) aqueous solution, anticoagulation of 2ml blood requires Desheng ethylenediaminetetraacetic acid dipotassium (EDTA-K) 4mg. Because the concentration is small, in order not to dilute the blood, 20ul of an aqueous solution containing 200g/L of dipotassium ethylenediaminetetraacetate (EDTA-K) is generally pre-placed in each blood collection tube. When the use environment changes slightly, such as temperature changes. Moisture is easily evaporated to the tube wall (especially the water in the plastic PET tube solvent can penetrate through the tube wall and leak out), causing EDTA-K in the tube to crystallize quickly, and the crystallized dipotassium ethylenediaminetetraacetate (EDTA) -K) When collecting blood, the blood collection tube must be turned upside down for at least 8 times, so that the crystallized dipotassium ethylenediaminetetraacetate (EDTA-K) can be fully dissolved and mixed in the blood. Hemolysis, platelet aggregation adhesion and fragmentation. Desheng is a professional manufacturer. The medical professionals in the chemical field have been recognized by many manufacturers for its quality. If you need to buy, please contact us!