Detection items and advantages of chromogenic substrate TOPS

Details:

Today's people, eating is no longer clear soup and water, and they will not be hungry and full. It is a fantasy to go to bed early and get up early. The happy life you think is actually slowly eroding our health step by step. Body. The most lively place in the hospital is the laboratory department. The common ones are the three highs, which are either liver problems or kidney problems. Various inspections become particularly important. Only by detecting where the problem is can we prescribe the right medicine and solve it as soon as possible. When it comes to the detection of liver and kidney, we have to talk about our display substrate TOPS.
TOPS for creatinine measurement of renal function:
In the presence of peroxidase, hydrogen peroxide reacts with 4-aminoantipyrine (4-AAP) and TOPS (ESPMT) or HTIB  to produce chromogenic products quinoneimines. Color intensity can be measured by absorbance and is directly proportional to creatinine concentration.
TOPS for uric acid detection of renal function:
The detection of uric acid is first to use uricase to catalyze the complete oxidation of uric acid with oxygen and water into allantoin, carbon dioxide and hydrogen peroxide H2O2. H2O2 reacts with TOPS and 4-AAP under the catalysis of POD, TOPS is oxidized into a color product, and the absorbance is measured with a spectrophotometer, and the absorbance is proportional to the content of uric acid, so as to conduct uric acid analysis.
TOPS is used for liver function free fatty acid detection:
Free fatty acids (NEFA) in serum samples react with ATP and coenzyme A (CoA) under the catalysis of fatty acyl-CoA synthase to generate fatty acyl-CoA (Acyl-CoA), AMP and phosphate (PPi). Acyl-CoA is oxidized to enoyl-CoA (Enoyl-CoA) and hydrogen peroxide under the catalysis of acyl-CoA oxidase, and finally hydrogen peroxide is catalyzed by peroxidase to react with 4-aminoantipyridine Lin and TOPS reacted to form a red quinoneimine compound.
TOPS advantages:
Because its molecular structure and the sulfopropyl group on the single atom connected to the benzene ring have one less hydroxyl group, the stability is slightly stronger, and it has the characteristics of high water solubility and high detection sensitivity. In the presence of hydrogen peroxide and peroxidase, the chromogen TOPS during the oxidative coupling reaction with 4-aminoantipyrine (4-AA) or 3-methylbenzothiazole sulfonehydrazone (MBTH), Forms very stable color dyes. It is widely used in diagnostic tests and biochemical tests, mainly for the colorimetric determination of uric acid and cholesterol; when it is used as a water-soluble reagent, hydrogen peroxide can be determined by enzymatic photometry.
TOPS storage requirements:
1. Its water solubility means that it must be operated in a closed environment and in a dehumidified environment, otherwise it is easy to absorb moisture and cause its chemical properties to change.
2. Pack this product in a light-proof bottle as it is sensitive to light.
3. The temperature should be kept at 0-5℃.