The difference and application of dipotassium and tripotassium ethylenediaminetetraacetate

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When it comes to EDTA, many people should have heard of it. Today, I will introduce two potassium salts used in blood coagulants. Let us first understand:
Dipotassium ethylenediaminetetraacetate, alias: dipotassium EDTA CAS number: 25102-12-9 Molecular weight: 404.46 Molecular formula: C10H18K2N2O10
Tripotassium EDTA, alias: Tripotassium EDTA CAS number: 65501-24-8 Molecular weight 442.54 Molecular formula: C10H13K3N2O8
Dipotassium EDTA is used as an additive for vacuum blood collection tubes in practice, as a toilet deodorant, as a complexing agent in liquid phase analysis, and as a metal polishing agent.
The practical application of EDTA tripotassium is used for complexing metal ions and separating metals, for preparing anticoagulants, anticoagulant tubes, and supporting whole blood analyzers; also used in detergents, liquid soaps, shampoos, agricultural chemical sprays Antidote, anticoagulant, blood anticoagulant, etc.
We see that both EDTA dipotassium and EDTA tripotassium are commonly used anticoagulants in blood collection tubes. The anticoagulation principle is to use EDTA to chelate Ca ions in the blood, thereby preventing the anticoagulation effect. Both are white crystalline powders, soluble in water and slightly soluble in alcohol, used to prepare anticoagulants, anticoagulant tubes, and complete blood analyzers. But we take a closer look and find that tripotassium has one more potassium ion than dipotassium.
Although both use blood collection tube anticoagulants, their anticoagulation capabilities are different, mainly in the ability to complex Ca ions. It is because of this that the difference in use is caused. % aqueous solution, the pH of EDTA-Ka2 is 4.8±1, and the pH of EDTA-Ka3 is 7.3±1. The difference is mainly reflected in the stable existence at different pH. EDTAK3 pH is neutral and does not affect the pH of blood. At the same time, its ability to complex calcium ions is stronger than that of EDTAK2, and its anticoagulant effect is stronger than that of EDTAK2; at the same time, the solubility of dipotassium is lower than that of tripotassium, which sometimes affects the anticoagulation effect; but if EDTAK3 is used as a liquid additive, it will lead to sample dilution. All directly detected items, such as hemoglobin, red blood cell, white blood cell, and platelet counts, were 1-2% lower than EDTAK2 as an anticoagulant. It seems that whether it is to choose dipotassium or tripotassium, there are advantages and disadvantages. The main thing is to choose the right one. We Hubei Desheng are a professional manufacturer of blood collection tube additives. We have been producing in this area for more than ten years, and the production process is mature. , the technical indicators are excellent, it is worthy of your trust!