Piperazine POPSO buffer

Details:

The Chinese name of POPSO is piperazine-N,N'-bis(2-hydroxypropanesulfonic acid), and the CAS number is 68189-43-5. In the range of weak alkalinity of 7.0-8.2, its buffering effect is the best, and It can keep the pH value of the solution in a stable state for a long time. The POPSO produced by our company is mainly reagent grade, which is often configured as a buffer solution for environmental research, chemical analysis and biological research to adjust and stabilize the pH of the research solution.
POPSO buffer and PIPES, HEPES, POPSO, HEPPS, HEPPSO, HEPBS buffer belong to the piperazine family series. This series of buffers all contain piperazine rings, which cannot react with various metals to form complexes and precipitate, but It can undergo redox reaction with strong acid and strong base, so it needs to be prepared and used when it is used. At room temperature, high-quality POPSO is a white crystalline powder with very stable chemical properties. 0.25g of POPSO powder can be completely dissolved in 1ml of sodium hydroxide and reach a saturated state, and the dissolved solution is clear to slightly turbid. POPSO powder is almost insoluble in other organic solvents, so it will not affect biochemical reaction experiments when used as a buffer.
In terms of molecular structure, POPSO is a dipole ionic compound with both positive and negative charges at the same time, that is, a zwitterionic compound. At 25 °C, the acidity coefficient of POPSO is 7.8, and it does not change greatly with temperature. Adding non-isoionic inorganic salts to POPSO buffer will not change the activity coefficient of the solution. In general, POPSO buffer
Does not penetrate biofilms. POPSO can be used in cell culture experiments that require metal cations, but cannot be used in mitochondrial studies, because POPSO has an inhibitory effect on mitochondrial anion one-way channels.
Desheng is a professional source manufacturer of POPSO. With the efforts of scientific researchers, our company has continuously improved the process of producing POPSO powder, which can ensure that the POPSO crystal powder produced is pure white with a purity of more than 99%. In 20%W/W sodium hydroxide solution, its absorbance of ultraviolet and visible light waves is less than 0.1. Our commitment to customers is always to deliver on time with quality and quantity. Welcome to consult.