Introduction and Application of Biological Buffer BIS-Tris Propane

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The Chinese name of BIS-TRIS propane is 1,3-bis(tris(hydroxymethyl)methylamino)propane, CAS No. 64431-96-5, the appearance is white crystalline powder, similar to TRIS buffer, because it is also TRIS series A kind of biological buffer, which is a derivative of TRIS. Let's follow the editor to see what features and applications it has.
Introduction to Bis-Tris Propane
Bis-Tris propane is a zwitterionic buffer mainly used in biochemistry and molecular biology. Because it has two pKa values ??of similar size, its first-order acid dissociation constant Pka1=6.8, and its second-order acid dissociation constant Pka2=9.0, it exhibits a very wide buffer range, about pH 6.3-9.5. It is precisely because of its broad buffer capacity that the pH can be as low as 6-7, making it more suitable than Tris buffer to improve the stability and activity of restriction endonucleases. It is also one of the most widely used reagents in the TRIS series.
Specific applications of BIS-TRIS propane
1. It can be used as a buffer in the study of DNA polymerase fidelity and PCR reaction, and the effect is good;
2. It is used as a buffer in the study of light-excited bacteriorhodopsin electrical signals;
3. For maintaining the stability of farnesyl pyrophosphate purified from Saccharomyces cerevisiae mutants;
4. For the study of enzyme activity in some experiments;
5. In protein for crystallization research;
6. It can be used as a buffer in other biochemistry and molecular biology.

Hubei Xindesheng Material Technology Co., Ltd. is a professional manufacturer of biological buffers. At present, the Bis-Tris propane produced has stable product performance and good use effect. It has been sold to many customers at home and abroad, and has been repeatedly praised. In addition to Bis-Tris propane, dozens of other biological buffers are also produced. You can find everything you want. If you need to purchase biological buffers, please contact us!