Principle and application of biological buffer Tris

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In biological experiments, biological buffers should be often used, and Tris is a common item in biological buffers. As a widely used biological buffer, many people should know this name, but some people do not know it. Here we can take a look at the principle and application of Tris.

Tris is called trihydroxymethylaminomethane, also known as aminobuttriol, CAS number is 77-86-1, the appearance of white crystalline powder. Soluble in ethanol and water, will also be dissolved with ethyl acetate but slower, not with ether, carbon tetrachloride, copper, aluminum corrosion.

Tris is widely used in acute metabolic and respiratory acidemia. As an alkaline buffer, Tris has a good buffering effect on metabolic acidosis and enzyme activity reactions, which may cause hypoglycemia, hypotension, nausea and vomiting. It can even inhibit breathing and cause respiratory arrest. Alkalinemia may occur when ingested in excess or when renal insufficiency occurs. When Tris and acid titration base materials are used, the purity requirements are high, ranging from 99.9% to 100%

Because Tris is weakly alkaline, its Pka is 8.1 when the temperature is below 25℃, but according to the buffering theory, its effective buffering range is between PH7.0 and 9.2. While the PH of Tris aqueous solution is about 10.5, hydrochloric acid is generally added to adjust the PH value to the desired value, but at the same time, attention should be paid to the influence of temperature on THE Pka of Tris. Since the Tris buffer is a weakly alkaline solution, DNA is deprotonated in the solution, increasing its solubility. Therefore, PEOPLE often add EDTA into Tris hydrochloric acid buffer to make TE buffer, and change the PH adjustment solution into acetic acid, TAE buffer can be obtained.

Desheng biotechnology has been established for decades, the research and development production of biological buffer has 15 years of experience, and Tris is the first research and development products of the company, as the company's core products, currently has more than one hundred countries in the world, reliable product quality, price concessions to talk about, are interested can inquire, DE sheng biotechnology welcome your calls.