Use of 4-hydroxyethylpiperazineethanesulfonic acid HEPES buffer

Details:

The full chemical name of HEPES buffer is 4-hydroxyethylpiperazine ethanesulfonic acid. Its main function is to maintain the pH value change caused by biological metabolic waste and control the pH environment within an appropriate value. The effective pH range of HEPES buffer It is 6.8-8.2, which can be used in cell experiments, bacterial culture, detection reagents, biological preservation solutions and other products.
1. Application of HEPES in cell culture
Cell culture is a cumbersome and demanding experimental technique. To enable cells to grow in vitro for a long time, the pH value of the growth environment is very demanding. There is a 3D three-dimensional cell culture system that can be used in cell experiments and bioengineering production. In its patent, HEPES is added to remove calcium ions and maintain the pH value of the medium, so that cells are relatively unaffected by the outside world. grow within the range.
2. HEPES is used for artificial insemination in animal husbandry
In animal husbandry, artificial insemination is a very important part, but after selecting the desired genes, there is a problem, because the sperm and eggs of animals are removed from the farm and transported to the laboratory. Difficult to save questions. After repeated experiments, it was found that adding an appropriate concentration of HEPES to the transport environment can prolong the survival time of the samples.
During this time, the survival rate of sperm and eggs is improved, and the probability of fertilization is greatly improved.
Hubei Xindesheng Material Technology Co., Ltd. is a professional manufacturer of biological buffers. The HEPES buffers currently produced are widely used and stable, and can be used in many fields. In addition, the company can also provide more than 30 kinds of biological buffers, blood collection tube additives, chemiluminescence reagents, color reagents, enzyme preparations, etc. If you need to buy, please contact us.