Chemical raw material Acid Blue 90 Powder CAS 6104-58-1 for Dye

Details:

Description of Acid Blue 90 from Wuhan Fortuna Chemical Co.,Ltd

Basic Information of Acid Blue 90
Product Name	Acid Blue 90
CAS NO.	6104-58-1
Assay	98 %min
Appearance	Dark blue powder
Pacakge	1kg/Foil Bag,25kg/Drum/Box
MOQ	1kg
Storage	Cool and dry place
Shelf Life	2 Years
Brand	Fortunachem

Specifications(COA) of Chemical raw material Acid Blue 90 Powder CAS 6104-58-1 for Dye

 

Introduction of Acid Blue 90

Acid Blue 90, also known as Coomassie Brilliant Blue R-250, is a synthetic dye commonly used in biochemical laboratories, particularly for staining proteins in various types of electrophoresis gels, such as SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis). Here are some key details about Acid Blue 90:

 

Chemical Information:

Chemical Formula: C45H44N3NaO7S2

 

Molecular Weight: 825.97 g/mol

 

CAS Number: 6104-58-1

 

Applications:

Protein Staining: Acid Blue 90 is widely used for staining proteins in polyacrylamide gels. The dye binds to proteins, allowing them to be visualized as distinct bands. This is particularly useful for analyzing protein purity, molecular weight, and concentration.

 

Bradford Protein Assay: Acid Blue 90 is a key component of the Bradford protein assay, a colorimetric assay used to measure the concentration of proteins in a solution. When the dye binds to proteins, it undergoes a shift in absorbance maximum, which can be measured spectrophotometrically.

 

Histology and Cytology: In addition to its use in protein analysis, Acid Blue 90 can also be used for staining tissues and cells in histological and cytological studies.

 

Properties:

Color: Acid Blue 90 is a dark blue dye.

 

Solubility: It is soluble in water and ethanol.

 

Binding: The dye binds to proteins primarily through ionic interactions and hydrophobic interactions, which makes it effective for staining purposes.

 

Advantages:

Sensitivity: Acid Blue 90 is highly sensitive and can detect small amounts of protein.

 

Ease of Use: The staining process is relatively straightforward and does not require complex procedures.

 

Reproducibility: The results obtained using Acid Blue 90 are highly reproducible, making it a reliable choice for protein analysis.

 

Procedure for Protein Staining:

Preparation: Prepare the staining solution by dissolving Acid Blue 90 in an appropriate solvent, often a mixture of methanol, acetic acid, and water.

 

Staining: After running the electrophoresis gel, immerse the gel in the staining solution and incubate for a specified period, usually a few hours.

 

Destaining: Remove excess dye by washing the gel with a destaining solution, typically containing methanol and acetic acid, until the background is clear and the protein bands are distinctly visible.

 

Safety:

Handling: As with all chemical reagents, handle Acid Blue 90 with care, using appropriate personal protective equipment (PPE) such as gloves and safety goggles.

 

Disposal: Dispose of the dye and any solutions containing it according to local regulations and guidelines for hazardous waste.

 

In summary, Acid Blue 90 (Coomassie Brilliant Blue R-250) is a versatile and widely used dye in biochemical research for staining proteins in electrophoresis gels and for protein quantification assays. Its high sensitivity, ease of use, and reproducibility make it an essential tool in protein analysis.

 

FAQ From Wuhan Fortuna Chemical Co.,Ltd
Q	Can I get a sample for free
A	For small QTY and low value product, we can provide sample for free exclude shipping cost. For high value sample, we will charge the basic sample cost, but we can refund all the cost if customers place order after sample test.
Q	How to confirm the product quality before placing orders
A	Most of our products confirm to international standards like USP/EP/BP/JP/CP. We will provide COA/TDS/MSDS for customers to confirm the specification before placing order.
Q	What is the payment you accept
A	For small and low value order, we accept CreditCard, MaterCard,VISA, and T/T.For large order, we can do T/T and L/C. Any pay on delivery or pay-after are not available for us.
Q	What is your shipment way
A	For less than 100kg gross weight order will ship with courier like FedEx or UPS, we support customers to place courier orders remotely by their account, we will package the goods well and wait for pick up.For more than 100kg goods, we will transport by Air or Sea according to customers' requirement.
Q	How long is your after-sales service
A	We accept customer’s quality complaint in three month after customer received the goods. We provide tech.support for all-life if customer place order from our company.
Q	How do you treat quality complaint
A	We support customer to provide our products to any rightful third party testing organization to test our products according to the COA specification, if the test result is confirm with COA, we will take all the loss for customers.

Wuhan Fortuna Chemical Co.,Ltd established in 2006, is a big integrative chemical enterprise being engaged in Pharmaceutical and Intermediates, Food and Feed additives, Fine chemicals,Biochemical and Reagent, which is  the members of Hubei E-commerce Chamber.

 

Our company has professional persons who major in chemical R&D and sicentific management, supply fine chemical products with high quality and close service, also supply custom-tailored products according to our clients' requirement.

 

We have a positive and self-motivated management work team with a common philosophy, caring and commitment through teamwork, our team strive to achieve success in delighting our clients and ourselves.

 

Since Wuhan Fortuna Chemical Co., Ltd founded, we continuously innovate our products and improve our service, sales network. Hence, we initiate the first sale mode on net in China, which is the retail trade of small package bring along wholesale of diversified management modes. Our products are exported widely to South Korea, Vietnam, Australia, Europe and South America, highly recommended by our clients.We insist on the management creed “Market is our compass, Quality is our life, Credit is our soul”. Clients’ trust is our forward power, their satisfaction is our struggling goal.

 

Wuhan Fortuna Chemical Co.,Ltd is a professional manufacturer which provide 1,4,7,10-Tetraazacyclododecane-1,4,7,10-Tetraacetic Acid with good quality and best price, welcome to contact us to get COA/TDS/MSDS of 1,4,7,10-Tetraazacyclododecane-1,4,7,10-Tetraacetic Acid.