Add time:07/22/2019         Source:sciencedirect.com

A rapid quantitative bioassay was developed which allows the measurement of trifolitoxin activity in 7 h. The previous assay required 2 days to observe trifolitoxin activity and was not quantitative. With this assay, we have defined a unit of trifolitoxin activity as that amount required to observe 50% inhibitors of growth of a 3-ml culture of Rhizobium leguminosarum bv. viceae 128C1 in 7 h. This assay was used to demonstrate that trifolitoxin and bacitracin have very different biological activities despite their similar structures. Minimum inhibitory concentrations of trifolitoxin were determined for several strains of Rhizobium, Bradyrhizobium and Agrobacterium.

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