Add time:07/23/2019         Source:sciencedirect.com

Trifolitoxin production by Rhizobium leguminosarum bv. trifolii T24 was observed in three sterile soils. The three soils differed in texture and fertility. Two of these soils were silt loams while the third soil was a silty sand. One of the silt loams was amended with manure and composted. The amount of trifolitoxin in these soils was determined by bioassay after inoculation with T24. The largest amount of trifolitoxin in each soil was observed at 48 h after inoculation; after 48 h, trifolitoxin declined. The amended soil contained 5–10 fold more trifolitoxin than did the unamended soils. The production of trifolitoxin in soil by T24 was verified in two ways: (i) T24 inoculated soils contain a substance which inhibits a trifolitoxin-sensitive strain of Rhizobium but not a trifolitoxin-resistant strain; and (ii) sterile soils inoculated with a Tn5 mutant of T24 that lacks trifolitoxin production (T24::Tn51) did not contain any detectable trifolitoxin activity during the 4 days of the experiment. These data demonstrate that trifolitoxin is produced by T24 in unamended as well as amended soils, although much larger amounts are detected in the amended soil.

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