Add time:07/24/2019         Source:sciencedirect.com

Gibberellin A20-13-O-glucoside (GA20-13-O-Glc) and GA8-2-O-Glc were identified by GC-MS as permethylated derivatives in mature grains of both barley varieties Hordeum vulgare L. cv. Himalaya and cv. Salome.Changes of the pool size of these GA conjugates together with those of the parent GA20 and GA8 were estimated during seed ripening by internal standard quantification. The pools of GA20 and GA8 were found to reach a maximum and then to decrease to small amounts at the stage of full ripeness. The amounts of GA20-13-O-Glc were higher at the beginning of ripeness and then declined until full ripeness but these changes were not as pronounced as for free GA20. Thus, in mature caryopses of both varieties cv. Himalaya and cv. Salome the content of GA20-13-O-Glc was three to four times higher than that of GA20. The pool of GA8-2-O-Glc increased dramatically during the final stages of ripening, in cv. Himalaya earlier than in cv. Salome, and reflected the intensive GA20 metabolism during ripening. This is the first report on the simultaneous quantification of the pools of GA-O-glucosides and their parent GAs during seed development on the basis of a GC-MS/internal standard technique.

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