Add time:08/01/2019 Source:sciencedirect.com
In order to improve the signal to noise ratio in ratiometric oxygen sensing, comparable phosphorescence and fluorescence intensities are required. To balance the room temperature phosphorescence (RTP) and fluorescence intensities, lutetium labeled hematoporphyrin monomethyl ether (Lu-HMME) was used and its luminescence and oxygen quenching properties were studied. Luminescence spectrum indicates that RTP and fluorescence intensities of Lu-HMME are in the same order of magnitude in air-saturated solution. As expected, the fluorescence emission of Lu-HMME was independent of dissolved oxygen while RTP emission was strongly dependent on oxygen. A compact and simple ratiometric oxygen measurement system based on Lu-HMME was constructed utilizing fiber optoelectronic sensing device. The intensity response (Initrogen/Ioxygen) of Lu-HMME RTP to dissolved oxygen is higher than 4.6. The measurement uncertainty of 0.06 μM in air-saturated methanol solution was achieved using Lu-HMME. Our results suggest a high accuracy method for ratiometric dissolved oxygen measurement based on balanced phosphorescence and fluorescence emissions.
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