Add time:08/09/2019 Source:sciencedirect.com
obtusifoliol (cas 16910-32-0)-[2,2,4-3H3] was synthesised and incubated with the chrysophyte alga Ochromonas malhamensis which converted it into poriferasterol. A reaction sequence applied to poriferasterol showed that the tritium retained at C-4 occupied the 4α-position. This demonstrates that biological C-4 demethylation of a 4α-methylsterol precursor by O. malhamensis results in the axial 4β-hydrogen being inverted into the equatorial 4α-position of the 4-desmethyl sterol product.
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