Add time:08/10/2019 Source:sciencedirect.com
Microsomes isolated from corn embryos (Zea mays) can demethylate the 14α-methyl group of obtusifoliol (cas 16910-32-0) 2. An enzymatic assay has been developped for obtusifoliol 14α-methyl-demethylase in higher plants. The enzymatic reaction was shown to occur sequentially, converting obtusifoliol 2 to 4α-methyl-5α-ergosta-8,24(28)-dien-3β-ol4 via the trienol 4α-methyl-5α-ergosta-8,14,24(28)-trien-3β-ol3 which was thoroughly identified. This enzymatic reaction is dependent of NADPH and molecular oxygen. It is inhibited by CO, menadione and specific inhibitors of cytochrome P-450, the CO inhibition being partially reversed by light. It is concluded that in Zea mays microsomes, obtusifoliol is demethylated at C-14 by a cytochrome P-450 containing monooxygenase system.
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