Add time:08/18/2019 Source:sciencedirect.com
A high-performance liquid chromatographic assay of α-amanitin and β-amanitin in human serum, urine, or stomach washings is described. Sample preparation involves a chemical step with deproteinization and organic solvent treatment, and a selective cleanup and concentration step on reversed-phase prepacked cartridges. Separations are performed on a reversed-phase analytical column under isocratic conditions with uv detection at 280 nm. The method allows the quantitation of α- and β-amanitin separately with a detection limit of 10 ng/ml for both toxins.
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