Add time:08/19/2019         Source:sciencedirect.com

Zh. Zhelev, A.Zheleva and K. Halacheva. Preparation of a β-amanitin - concanavalin A conjugate of low toxicity. Toxicon25, 981 – 987, 1987.—Pure β-amanitin was isolated by combined adsorption chromatography of a crude methanol extract from Amanita phalloides on Sephasorb HP Ultrafine and Sephadex LH-20. The β-amanitin was coupled to concanavalin A by the carbodiimide method. The conjugate was purified by fractionation on a column of Sephadex G-75. The molar ratio β-amanitin to concanavalin A in this conjugate was 4.14, The purified conjugate was tested by thin-layer chromatography and showed characteristic (for amatoxins) bright purple staining but different mobility. The ultraviolet spectrum of the conjugate was different from the spectra of the native β-amanitin and the native concanavalin A. The toxicity of the conjugate was determined by in vivo toxicological studies and was four-fold lower than that of the native β-amanitin. These results suggest that this conjugate may be used for immunization procedures and for the production of amatoxin-specific antibodies.

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