Add time:08/20/2019         Source:sciencedirect.com

This paper assesses the application of pulsed electric fields (PEF) to the fresh biomass of Artrhospira platensis in order to enhance the extraction of C-PHYCOCYANIN (cas 11016-15-2) into aqueous media.Electroporation of A. platensis depended on both electric field strength and treatment duration. The minimum electric field intensity for detecting C-phycocyanin in the extraction medium was 15 kV/cm after the application of a treatment time 150 μs (50 pulses of 3 μs). However higher electric field strength were required when shorter treatment times were applied. Response surface methodology was used in order to investigate the influence of electric field strength (15–25 kV/cm), treatment time (60–150 μs), and temperature of application of PEF (10–40 °C) on C-phycocyanin extraction yield (PEY). The increment of the temperature PEF treatment reduced the electric field strength and the treatment time required to obtain a given PEY and, consequently decreased the total specific energy delivered by the treatment. For example, the increment of temperature from 10 °C to 40 °C permitted to reduce the electric field strength required to extract 100 mg/g dw of C-phycocyanin from 25 to 18 kV/cm, and the specific energy input from 106.7 to 67.5 kJ/Kg.Results obtained in this investigation demonstrated PEF's potential for selectively extraction C-phycocyanin from fresh A. platensis biomass. The purity of the C-phycocyanin extract obtained from the electroporated cells was higher than that obtained using other techniques based on the cell complete destruction.

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