Add time:08/22/2019         Source:sciencedirect.com

The low polarity of Carcinolopin and its positive reaction to colour tests for sterols indicated that this substance might be a sterol ester. Cholesterol esters separated from beef liver lipids by silicic acid chromatography showed a stimulating effect on the incorporation of labelled algal protein hydrolysate into soluble RNA of rat liver in vitro. A group of cholesterol esters with saturated fatty acids was separated from cholesterol oleate and palmitoleate by elution with carbon tetrachloride from silicic acid columns. Only the saturated fraction had a protein synthesis-stimulating effect. A reversed-phase liquid-liquid chromatographic technique was developed for the fractionation of individual esters with saturated fatty acids. 10% Apiezon M grease on Celite 545 was used as stationary phase and the esters were eluted with acetone in methanol by an ascending technique. Only one fraction, containing a fatty acid with carbon number 16.7 as the main component, was active. It seems that carcinolipin (cas 19477-24-8) is an ester of cholesterol with a branced-chain fatty acid.

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