Add time:08/21/2019         Source:sciencedirect.com

A procedure is described for the quantitative determination of Carcinolipin (cholesterol 14-methylhexadecanoate) in 0.5-1 g samples of biological materials (blood serum and tissues). The material is extracted with chloroform-methanol 2:1, internal standards of cholesterol margarate and 14-methylhexadecanoate are added and cholesterol esters separated by thin layer chromatography on silicic acid using petroleum ether-ether 95:5 for the development. Cholesterol esters are hydrolyzed; the free fatty acids extracted, esterified with diazomethane and separated by gas-liquid chromatography. The quantity of Carcinolipin present is calculated on the basis of peak heights of methyl margarate and 14-methylhexadecanoate. The method described shows a good reproducibility and may be used for the determination of 2.5–100 μg of Carcinolipin in the sample.

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