Add time:08/23/2019         Source:sciencedirect.com

An accurate, simple, reproducible and sensitive method for the determination of atropine sulfate and Scopolamine hydrobromide (cas 114-49-8) has been developed and validated. Atropine sulfate and scopolamine hydrobromide were separated using a μBondapack C18 column by isocratic elution with flow rate 1.0 ml/min. The mobile phase composition was methanol, water, formic acid (165:35:1; v/v/v) and pH adjusted 8.3 with triethylamine. The samples were detected at 230 nm using photo-diode array detector. The linear range of detection for atropine sulfate (I) and scopolamine hydrobromide (II) were between 10.38 and 1038 μg/ml with a limit of quantification (LOQ) of 10.38, 10.00 and 1034 μg/ml with an LOQ of 10.00 μg/ml respectively. The linearity, range, peak purity, selectivity, system performance parameters, precision, accuracy, robustness and ruggedness for (I) and (II) were also shown acceptable values.

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