Add time:08/26/2019 Source:sciencedirect.com
Antibiotic contamination to the environment is a global problem now a days, due to the prevalence of resistance. The antibiotic resistant Serratia sp. R1 was isolated from Yamuna river water to check its antibiotic degradation potential. Bacteria was characterized by 16s-rRNA gene sequencing, bacteria taxonomy and Phylogenetic tree was constructed by EZTaxon and MEGAX. Metabolic potential of bacteria was checked, and up to 84% degradation of Penicillin G was observed by Ultra performance liquid chromatography. Change in bacterial protein profile and comparable three protein bands was observed and analyzed by MALDI/TOF MS. The identified protein bands showed similarity with transposase, Type II secretion system F family protein and Esterases. In-silico study of enzymes also reveals the 3D structures and active amino acids, involved in the interaction with the antibiotic. Hence, the enzymatic product of Serratia sp. R1 may be a significant contributor in the bioremediation of Penicillin G for clean water resources and safer environment.
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