Add time:09/04/2019         Source:sciencedirect.com

Using B1 chain of β-bungarotoxin (β-Bgt) as bait in yeast two-hybrid screen, we found that KChIP3 was a binding protein of B1 chain. Thus, protein–protein interaction between β-Bgt and KChIP3 is investigated in the present study. Pull-down assay showed that recombinant KChIP3 proteins were associated with β-Bgt as well as B1 chain, whereas the inability of KChIPs 1, 2 and 4 to bind with β-Bgt was observed. Although Ca2+ was not a crucial factor essential for the binding of KChIP3 with β-Bgt and B1 chain, their interaction could be enhanced by the addition of Ca2+. Alternatively, the association of A1 chain of β-Bgt with KChIP3 was marginally detected. The dissociation constant of β-Bgt with KChIP3 were 12.2 and 6.08 μM in the absence and presence of 2 mM Ca2+, respectively. Moreover, native KChIP3 from rat brain was to be isolated by β-Bgt-Sepharose. These observations indicate that KChIP3 is a binding protein of β-Bgt. In view of the multiple functions of KChIP3 in neuronal cells, the interaction of KChIP3 with β-Bgt may represent an event for the manifestation of the biological activities of β-Bgt.

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