Add time:09/05/2019         Source:sciencedirect.com

In the present study, Xenopus nerve–muscle cultures were used to explore the functional roles of A chain (a phospholipase A2 subunit) and B chain (a non-phospholipase A2 subunit) of Bungarus multicinctus β-bungarotoxin. It was found that β-bungarotoxin induced an increment of the frequency of spontaneous synaptic currents (SSCs) in the nerve–muscle cultures. Modification of β-bungarotoxin with pyridoxal-5′-phosphate or substitution of Ca2+ with Ba2+ in buffer abolished the phospholipase A2 activity of β-bungarotoxin and the facilitatory phase of SSC as well. Antibodies that were directed specifically against A chain or B chain effectively inhibited phospholipase A2 activity, and as a consequence the SSC frequency was not greatly different from the control rate. These results suggest that both A and B chains are indispensable parts of β-bungarotoxin for inducing the facilitation of SSC frequency with Xenopus nerve–muscle cultures.

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