Add time:08/31/2019         Source:sciencedirect.com

KB cells growing exponentially in suspension were infected with type 2 adenovirus at high input multiplicities. An increase in intracellular virus was first detected at 14 hours after infection and reached levels of 4000–10,000 PFU per cell at 36–48 hours after infection. Uninfected cells multiplied 3- to 4-fold during 48-hour experimental periods. In contrast, multiplication of cells in infected cultures was limited to the first 12 hours and was never more than 1.2- to 1.3-fold. Measurements of virus adsorption, infective center formation, and surviving cells indicated that essentially all cells were infected within 1 hour after addition of virus. Infected cells did not lyse but appeared to increase in size. Dry weight analysis revealed a continuous increase in mass per infected cell to double that of control cells at 36 hours after infection. Protein, DNA, and RNA accumulated continuously in infected cells starting within 12 hours after infection. At 36 hours after infection the content per cell of these polymers reached double that of uninfected cells. The significance of increased polymer synthesis in relation to virus production is discussed.

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