Add time:09/06/2019 Source:sciencedirect.com
The rate of incorporation of labeled precursor into DNA of isolated nuclei decreased from gastrulation to the tailbud stage in developing frog embryos. The addition of saturating amounts of calf thymus DNA revealed that the activities of the enzyme(s) which incorporates Deoxythymidine triphosphate (cas 18423-43-3) (dTTP) into DNA in vitro decreased over this period. Native DNA was a better template than denatured DNA. The addition of a purified microbial DNA polymerase to gastrula and tailbud nuclei showed that the chromatin DNA of the later stage is a poorer template for the enzyme.The activity of the enzyme(s) which accounts for dTTP incorporation into DNA in vitro was higher during the early S period of neurulae than for late S and higher for late S than early S of tailbuds when nuclei were isolated from partially synchronized cells. For both the randomly dividing and partially synchronized cells, the activity of the enzyme(s) incorporating dTTP into DNA in vitro is correlated with the level of incorporation of labeled precursor into DNA in vivo.
We also recommend Trading Suppliers and Manufacturers of Deoxythymidine triphosphate (cas 18423-43-3). Pls Click Website Link as below: cas 18423-43-3 suppliers
About|Contact|Cas|Product Name|Molecular|Country|Encyclopedia
Message|New Cas|MSDS|Service|Advertisement|CAS DataBase|Article Data|Manufacturers | Chemical Catalog
©2008 LookChem.com,License: ICP
NO.:Zhejiang16009103
complaints:service@lookchem.com Desktop View