Add time:09/07/2019         Source:sciencedirect.com

A simple and rapid method has been developed for the isolation of thermally labile DNA alkylation products. Use of calf thymus DNA in combination with size exclusion filtration provides good yields >40% of chlorambucil-guanine adducts. Application of this method to the isolation of alkylation products of carzinophilin/azinomycin B affords material consistent with guanine monoalkylation.

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