Add time:09/05/2019         Source:sciencedirect.com

Drugs may be metabolised to reactive electrophilic species that spontaneously react with proteins. The presence of such drug–protein adducts has been associated with drug toxicity. In this study, the reactivity of the major metabolite of naproxen—the 1-β-O-glucuronide (Nap-GlcU)—was compared to the corresponding naproxen coenzyme A (Nap-CoA) thioester. The reactivity of the two metabolites was assessed in vitro in a phosphate buffer (pH 7.4; 0.1 M) at 37 °C towards the model bionucleophiles glutathione and human serum albumin (HSA). The reaction between the electrophilic species (Nap-GlcU and Nap-CoA) and glutathione forming the Nap-glutathione conjugate was monitored using LC–MS–MS and LC–UV, respectively. It was shown that Nap-CoA resulted in an approximate 100-fold higher formation of Nap-glutathione conjugate than Nap-GlcU. The presence of Nap-CoA also resulted in acylated HSA with a rate and a yield that was significantly higher than reported for Nap-GlcU. In summary, the data suggest that CoA metabolites may be more reactive species than acyl glucuronides that previously have been associated with severe drug related side effects in vivo.

We also recommend Trading Suppliers and Manufacturers of COENZYME A TRILITHIUM SALT (cas 18439-24-2). Pls Click Website Link as below: cas 18439-24-2 suppliers