Add time:09/06/2019 Source:sciencedirect.com
A new process has been described for the preparation of coenzyme A of high purity. Pantothenic acid, cysteine and AMP when added to cultures of Brevibacteriumammoniagenes IFO 12071 gave coenzyme A in a high yield. The product was obtained by using Duolite S-30, charcoal and Dowex 1-X2. This method is simple, rapid, compact, and requires no special equipment. It has been shown to be adaptable for preparing large amounts of highly pure coenzyme A. Similarly, pantothenic acid 4′-phosphate and pantetheine 4′-phosphate were readily obtained in good yields.
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