Add time:09/05/2019         Source:sciencedirect.com

The first synthesis of highly fluorescent acridine-1,8-dione dye labeled aminoallyl-uridine-5′-triphosphate is described. The coupling reaction of acridine-1,8-dione benzoic acid succinimidyl ester with aminoallyl-uridine-5′-triphosphate, followed by anion exchange purification results in the final compound with good yield and high purity. The acridine-1,8-dione benzoic acid succinimidyl ester is obtained by the reaction of acridine-1,8-dione benzoic acid with N-hydroxysuccinimide. This fluorogenic acridine-1,8-dione dye substituted uridine nucleotide has potential to generate labeled nucleic acids for molecular biology and molecular cytogenetic applications.

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