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  • Amino Acids | Thin-Layer (Planar) Chromatography☆
  • Add time:09/05/2019         Source:sciencedirect.com

    Thin-layer chromatography (TLC) is a simple and inexpensive technique permitting a number of samples to be handled simultaneously, thus yielding a higher precision than sequential analysis. The inert character of the thin-layer material makes it ideally suitable for use with strong corrosive reagents and one can perform many kinds of chemical reactions on the plate, both from the points of view of detecting and locating the spot and of achieving improved separation. Certain groups of interest can be chemically bonded to the reactive groups of support material, for example silanization for reversed-phase studies. Impregnation of the adsorbent with a variety of reagents adds an additional feature for influencing the adsorption characteristics without covalently affecting the inert character of the adsorbent. TLC is also successful in providing direct resolution of enantiomers of a variety of compounds by the proper manipulation of the support material. The analysis of amino acids, and their derivatives, the resolution of enantiomers of amino acids by TLC techniques using a wide variety of adsorbents and impregnating agents, the enantioresolution of racemic compounds using amino acids as impregnating agents, the possibility of obtaining relationships between the chromatographic behavior and chemical structure and the many practical applications drawn from the literature are described in detail in the following sections.

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    Prev:Chapter 20 - Trihydric Alcohols and their Oxidation Products (continued) Dihydroxycarboxylic Acids and Trihydroxycarboxylic Acids and Related Compounds (second part)
    Next: Purification, characterization and molecular cloning of an acidic amino acid-specific proteinase from Streptomyces fradiae ATCC 14544)

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