Add time:09/25/2019 Source:sciencedirect.com
The importance of phosphorus compounds in metabolism has been established by the work of many investigators. Neuberg and others have shown that rather simple, naturally occurring phosphates can solubilize many physiologically important substrates. A detailed study of the sodium salt of d(−)-3-phosphoglyceric acid (Na2PGA) has been made. This salt has been shown to be a good solubilizing agent for many water-insoluble salts, such as calcium carbonate. The eight most common metals of biological significance: calcium, cobalt, copper, iron, magnesium, manganese, nickel, and zinc were selected for detailed studies. Using the enzyme phosphatase it has been shown that once a soluble PGA complex is formed, it can be hydrolyzed to liberate phosphate under physiological conditions of pH and temperature. The rates of decomposition for all salts studied were quite similar. Spectrophotometric analysis of the complexes formed indicated a 1:1 combining ratio for calcium, copper, nickel, and manganese with PGA. Cobalt formed a double salt and magnesium a triple salt, whereas the ferric ion seemed to form several types of complex salts. Magnetic measurements proved the nickel complex to be square planar.
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