Add time:10/01/2019 Source:sciencedirect.com
The aim of this study was to investigate anesthetic efficacy of Myrcene (cas 123-35-3) and linalool on Cyprinus carpio in comparison with eugenol. The fish were exposed to 150, 200, 400, 600, 800, 1000 and 1200 ppm myrcene, 200, 400, 600, 800, 1000, 1200, 1400 and 1600 ppm linalool, and 25, 35, 50, 75, 100, 150 and 200 ppm eugenol and induction time were recorded based on fish behaviors. Exposure of the fish to 150–1000 ppm myrcene led to 594–42.7 s induction time with no significant difference between 1000 and 1200 ppm. Recovery at 150–800 ppm myrcene occurred within 149–272 s with no significant difference among 800–1200 ppm. Exposure to 200–1400 ppm linalool resulted in 812–79 s induction time, with no significant difference between 1400 and 1600 ppm. There was no significant difference in recovery time at different linalool concentrations (150–165 s). Induction time in both anesthetics were significantly dependent on anesthetic concentrations (R2 = 0.94–0.96). Recovery time in the myrcene-exposed fish was dependent on anesthetic concentrations (R2 = 0.77). Using regression models, calculated myrcene concentrations to induce stage 4 anesthesia within 60 and 180 s were 819 and 350 ppm. Required linalool concentration to induce stage 4 anesthesia within 180 s was calculated to be 753 ppm. Myrcene at 50 ppm and linalool at 50 and 100 ppm are able to keep the fish at stage 2 anesthesia for 2 h. This study showed for the first time that linalool and myrcene are potential anesthetic in carp, although not as efficacious as eugenol.
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